Ph-ncas9-ppe
Webb高彩霞团队改造并比较了三组植物引导编辑系统（plant prime editor systems，PPEs），它们分别为PPE2,PPE3和PPE3b。 其中，PPE2对应上文刘如谦团队的PE2，包含融合逆转录酶M-MLV-RT的nCas9（H840A），和一个包含PBS和反转录模板的pegRNA。 PPE3对应于上文的PE3，在PPE2的基础上添加了一个可以靶向并指导切割非编辑链sgRNA。 PPE3b则 … WebbLocation : Home > Faculty > Caixia Gao > Selected Publications. Selected Publications. Publications (* Corresponding author; 1 co-first author) 1. Wang S1, Zong Y1, Lin Q1, Zhang H1, Chai Z, Zhang D, Chen K, Qiu JL, Gao C * (2024) Precise, predictable multi-nucleotide deletions in rice and wheat using APOBEC-Cas9.
Ph-ncas9-ppe
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WebbpH-nCas9-PPE-V2 pSpG-PPE pH-ePPE ePPE pRN120 Sign Up for Our Newsletter. Receive the latest news, hot plasmids, discounts and more. Sign Up. Subscribe to Our Blog. Learn … WebbLin Q, Zong Y, Xue C, Wang S, Jin S, Zhu Z, et al.Prime genome editing in rice and wheat. Nat Biotechnol. 2024;38:582-585 pubmed publisher
WebbpH-nCas9-PPE-V2 Plasmid #170131 Purpose For plant prime editing in rice plants or monocotyledons protoplasts Depositor Caixia Gao Article Lin et al Nat Biotechnol. 2024 Mar 25. pii: 10.103 Insert nCas9 (H840A)-M-MLV Use CRISPR Tags Expression Plant Mutation H840A for Cas9; D200N, T306K, W313F, T330P and L… Promoter maize … http://www.biofeng.com/adjy/zhili/pH-nCas9-PBE.html
WebbpH-nCas9-PBE.gb A portion of this plasmid was derived from a plasmid made by APOBEC1, XTEN, nCas9 (D10A), and UGI sequences were codon-optimized for cereal … WebbpH-nCas9-PBE DescriptionAlias:L-98163 Promoter:OsU3,UBI Replicator:pVS1 Clone strain:Stbl3 Culture conditions:37centigrade Host
WebbPlasmid pH-nCas9-PPE from Dr. Caixia Gao's lab contains the insert nCas9(H840A)-M-MLV and is published in Nat Biotechnol. 2024 May;38(5):582-585. doi: 10.1038/s41587-020 …
Webb21 feb. 2024 · Cas9 protein (1 μg) and sgRNA (1 μg) were mixed with the purified target DNA (100–150 ng) in Cas9 reaction buffer (20 mM HEPES, pH 7.5, 150 mM KCl, 10 mM MgCl 2, 0.5 mM DTT) in a total volume of 20 μl, followed by digestion at 37 °C for 0.5–1 h. grand budapest hotel movie based on what bookWebb7 jan. 2024 · We designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein. origin : 37 pi : resistance … chin-chin cafe chinese thai sushi \\u0026 cateringgrand budapest hotel movie run timeWebbpOsU3 pTaU3 pH-nCas9-PPE-V2/pH-ePPE Forward primer (5'-3'): + Spacer sequence + Reverse primer (5'-3'): + PBS and RT template sequence + Submit >> clear. Help … grand budapest hotel photographyWebbPlasmid pSpG-PPE from Dr. Caixia Gao's lab contains the insert nCas9-SpG(H840A)-M-MLV and is published in Nat Biotechnol. 2024 Mar 25. pii: 10.1038/s41587-021-00868-w. doi: … chin chin cheersWebbThis plasmid was modified from pKDL071 described in Litcofsky K.D., Afeyan R.B., Krom R.J., Khalil A.S., Collins J.J.; Iterative plug-and-play methodology for ... chin chin carlton northWebb7 jan. 2024 · product information. Strains containing DP6 must be maintained in rich media supplemented with 25 mM glucose. Received from Ahmed Badran, former Liu lab member. chin chin chinese