Web12 apr. 2024 · Oxygen consumption was determined using a Seahorse XF cell Mito Stress Test kit (Agilent, USA) according to the manufacturer’s instructions. Briefly, HepG2, Huh7, and MHCC97H cells were infected with the lentivirus shHIGD2A or shCtrl and seeded onto Seahorse cell culture microplates at a density of 10,000, 7200, and 9000 cells/well, … WebHi Laura, Thank you for contacting Cell Analysis Technical Support. In general, It is not recommended to use ECAR data from a Cell Mito Stress Test (MST) to assess glycolytic activity for this primary reason: ECAR in the MST is not a direct measurement of glycolysis, but rather a summation of acid (H +) produced from glycolysis and/or the TCA cycle (via …
Chemical Analysis, Life Sciences, and Diagnostics Agilent
Web3 mrt. 2024 · The Cell Mito Stress Test is a common method to measure the key parameters of mitochondrial respiration. Here, we use the human cell line HK-2 as an example to present the procedures to quantify the oxygen consumption rate using a Seahorse XFe96 extracellular flux analyzer. WebThe Seahorse XF Cell Mito Stress Test can help quantify multiple parameters of mitochondrial respiration and identify mitochondrial dysfunction. See what … the glory of japan
Extracellular Flux Assays to Determine Oxidative ... - SpringerLink
Web• Load the assay cartridge with XF Cell Mito Stress Test compounds (final concentrations: 2.5 μg/mL oligomycin, 0.8 or 1.6 μM FCCP, 2 μM rotenone/4 μM antimycin A.). Web1 sep. 2015 · The general scheme of mitochondrial stress test is shown in Fig. 3A. Sequential injections of oligomycin, FCCP, rotenone and antimycin A measure basal respiration, ATP production, proton leak, maximal respiration, spare respiratory capacity, and non-mitochondrial respiration. WebAgilent Seahorse Mito Stress Test Single-File and Mulit-File XF Report Generator FAQs. Agilent Seahorse Motor error/plate tray immovable: Troubleshooting Tips. ... The XF Sensor Cartridge must be hydrated for at least 4 hours before your XF … the glory of innovation