Hoechst 33342 structure

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August undefined, 2024

NettetHoechst 33,342 staining EMT-6 cells incubated with varying concentrations of Rh4 for 24 h were cleaned with PBS, followed by fixing with 4% paraformaldehyde for 20 min. Then, images were taken with a fluorescence microscope (Nikon, Japan) after 20 min of staining with Hoechst 33,342 (Solarbio, Beijing, China). NettetFig. 1. Molecular structure of PureBlu Hoechst 33342 Nuclear Staining Dye. CI C 3 H 3 C N N N O NH NH N H 2 N H CI N CI H 2 N HCI N H CI N H Fig. 2. Excitation and emission spectra of PureBlu Hoechst 33342 Dye. Normalized intensity 100 80 60 40 20 0 300 400 500 600 700 Wavelength, nm PureBlu Hoechst 33342 Nuclear Staining Dye …

Protocol: Staining Cells with Hoechst or DAPI Nuclear Stains

NettetInvitrogen Hoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available as a solution (Cat. No. H3570). NettetHoechst 33342 nucleic acid stain is a popular cell-permeant nuclear counterstain that emits blue fluorescence when bound to dsDNA. This dye is often used to distinguish condensed pycnotic nuclei in apoptotic cells and for cell cycle studies in combination with BrdU. It is also available in solution (H-3570). mek therapie

Hoechst 33342 Solution (20 mM)

NettetFeatures of Hoechst 33342 Fluorescent Stain: • Hoechst dye—blue fluorescent stain specific for DNA (i.e., nuclei of eukaryotic cells) • Convenient—provided as an easy-to … Hoechst 33342 is used for specifically staining the nuclei of living or fixed cells … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes These dyes can also stain Gram-positive and Gram-negative bacteria. There are … The Thermo Scientific FITC Antibody Labeling Kit is a multi-component kit … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes NettetIts uses are similar to Hoechst 33342 (H-1399) for counterstaining, apoptosis and cell cycle studies, but Hoechst 33258 is reportedly less cell-permeant. For Research Use Only. Not for use in diagnostic … napa valley wine inn

Hoechst 33342 - Handbook of Fluorescent Dyes and Probes

Hoechst 33342 CAS#:23491-52-3 Chemsrc

NettetA chemical structure of a molecule includes the arrangement of atoms and the chemical bonds that hold the atoms together. The Hoechst 33342 molecule contains a total of … NettetStructure Search. Search Within. Products Technical Documents Site Content Papers Genes Chromatograms. Product Category. dyes (2) Brand. Sigma-Aldrich (4) Formula Weight. Physical Form. ... red nuclear staining dye with greater photostability than traditional blue fluorescent nuclear stains such as DAPI and Hoechst 33342. meksyk all inclusiveNettetBisbenzimide Hoechst 33342 is a specific stain for AT-rich regions of double-stranded DNA and has been shown to displace several known DNA intercalators. This fluorescent dye has been used in sorting living cells based on DNA content, used in flow cytometry for the determination of DNA content, and for the visualization of chromatin distribution in … mek surface tension

"Nettet5. nov. 2015 · The fluorescent dye Hoechst 33342 is a substrate for many multidrug transporters and is extruded by efflux pumps in microbial and mammalian cells. ... Alemán C, Namba AM, Casanovas J. Acid-base and electronic structure-dependent properties of Hoechst 33342. J Biomol Struct Dyn 2005; 23: 29–36. pmid:15918674 . View Article " - Hoechst 33342 structure

Hoechst 33342 structure

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NettetFind hoechst 33342 and related products for scientific research at Merck. NL EN. Applications Products Services Support. Advanced Search. Structure Search. Search Within. Products Technical Documents Site Content Papers Genes Chromatograms. Product Category. dyes (4) Brand. Sigma-Aldrich (6) Formula Weight. Physical Form. … NettetIt has been shown that polarity of environment around Hoechst 33342 is almost unchanged while sorption of this fluorescent dye on a surface of the hairpin oligonucleotide HP1, t-RNA and DNA. At small concentrations, ... Molecular structure of d(CGC- [e6G]AATTCGCG complexed with Hoechst 33258 or Hoechst 33342. The EMBO …

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A concentration of 0.1–12 μg/ml is commonly used to stain DNA in bacteria or eukaryote cells. Cells are stained for 1-30 min at room temperature or 37 °C and then washed to remove unbound dye. A green fluorescence of unbound Hoechst dye may be observed on samples which are stained with too much dye or which are washed partially. Hoechst dyes are often used as substitutes for … Nettet26. nov. 2012 · The Hoechst 33342 dye is similar to DAPI in that both are UV-excited, minor groove-binding, and emit signals proportional to total DNA content. Both are maximally excited around 355 nm and emit around 460 nm. A UV light source is required, which may harm the cell.

NettetbisBenzimide H 33342 trihydrochloride. Synonym (s): 2′- (4-Ethoxyphenyl)-5- (4-methyl-1-piperazinyl)-2,5′-bi-1H-benzimidazole trihydrochloride, HOE 33342, Hoechst 33342, … Nettet20. feb. 2015 · Other properties listed for this fluorescent dye include the physical form, solubility, melting point, boiling point, pKa, absorption maxima, emission maxima, molar extinction coefficient and quantum yield. The chapter also includes references on the use, properties, and safety/toxicity of Hoechst 33342 on humans, animals and the …

Nettet18. apr. 2024 · Compared to Hoechst 33258, Hoechst 33342 is significantly more cell-permeable due to the addition of a lipophilic ethyl group and is usually preferred for … NettetHoechst 33342 is a bis-benzimide derivative that binds to AT-rich sequences in the minor grove of double-stranded DNA. The emission is very bright, and for the purposes of the …

NettetHoechst 33258, pentahydrate (bis-benzimide) (MW 623.96) (H3569) 10 mL 10 mg/mL (16.0 mM) solution in water Store at 2–6°C Protect from light • • When stored as directed, product is stable for 6 months. Hoechst 33342, trihydrochloride, trihydrate, FluoroPure™ grade (MW 615.99) (H21492) 100 mg NA ≤25°C Protect from light • •

NettetBisbenzimide C27H28N6O CID 1464 - structure, chemical names, physical and chemical properties, classification, patents, literature, biological activities, … mek test procedure for powderNettetBlue = Hoechst 33342 (fluorescent DNA dye). Show Less Show More. Flow cytometric analysis of Jurkat cells using Phospho-Histone H3 ... modify, reverse engineer, decompile, disassemble or otherwise attempt … napa valley wine priceNettet26. nov. 2012 · The Hoechst 33342 dye is similar to DAPI in that both are UV-excited, minor groove-binding, and emit signals proportional to total DNA content. Both are … mek the christmas catch in a good moodNettet20. feb. 2015 · This chapter lists the CAS registry number, chemical structure, chemical abstract (CA) index name, other chemical names, Merck Index number, chemical/dye … mek the bakerNettet10. aug. 2016 · 2D structures of Hoechst 34580 and Hoechst 33342 Full size image ThT assay Aβ42 fibrillization was measured using a Thioflavin T (ThT) assay. The compounds, from 1.22 μM to 10 mM, were prepared in DMSO and 0.4 μl of each was added to 384 well black plate. Each concentration was prepared in independent triplicates and a solvent … mekton zeta advanced technical manualNettetHoechst 33342 is a well-characterized blue-emitting fluorescent compound widely utilized for nuclear staining. Hoechst 33342 permeates cell membranes and binds to the minor groove of A/T-rich dsDNA sequences, thus preferentially staining nuclei (Figure 1). Fig.1. Hoechst 33342 (in blue) binding dsDNA. Hoechst 33342 Excitation / Emission Spectra mekthorg the wild wowNettetThe Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used … mektig technology group